EKC2023

[P33-LH]Screening monoclonal antibodies to recognize pathogenic microorganisms

Not scheduled

20m

Poster Poster(Wed)

Speaker

Jieun JANG (Korea Research Institute of Bioscience and Biotechnology)

Description

Pathogenic microorganisms with harmful toxins can cause fatal effects and infectious diseases in humans. The concern is that there is a risk of easy exposure to bacteria such as Bacillus anthracis, Yersinia pestis, Staphylococcus aureus, etc. The development of diagnostic antibodies is clinically important in that biological agents associated with infectious pathogens can be diagnosed easily and accurately. Through a Baculovirus Expression Vector System (BEVS), we first produced antigens of bacterial pathogens to develop monoclonal antibodies (mAb) to be used in a sensitive immunodetection system. BEVS is a suspension-cultured method using insect cells, and is useful for the production of recombinant proteins with high production reproducibility and unified refining conditions. In addition to hybridoma system, which has been widely used for antibody screening, we intend to build an immune-antibody library with more than 107 diversity and obtain highly-specific antibodies through phage display panning. High-specific antibodies were selected by securing hybridoma and immune libraries in mice immunized with pathogenic proteins. Here we discovered anti-Staphylococcal enterotoxin B (SEB) mAb. BEVS showed usefulness in producing high-purity recombinant SEB with maintaining immunogenicity at high yield. The four discovered highly specific antibodies and their combinations were provided in immunodetection kits and showed higher sensitivity in SEB detection than in commercially available kits. In addition, immunodetection analysis has been shown to be sensitive to reliable levels of routine identification in various samples, including food.

References

Alouf, J. E., & Müller-Alouf, H. (2003), Staphylococcal and streptococcal superantigens: molecular, biological and clinical aspects., International journal of medical microbiology. 292(7-8), 429-440.
Perry, R. D., & Fetherston, J. D. (1997), Yersinia pestis--etiologic agent of plague. Clinical microbiology reviews, 10(1), 35-66.
Zavialov, A. V., et al. (2003), Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Cell, 113(5), 587-596.