Speaker
Description
The need for an accurate and rapid diagnosis platform for highly contagious virus such as pandemic virus has been increased rapidly. At the initial stage of quarantine, conventional diagnostic method for the prevention of epidemic confronted those limitations such as long operation time, occupation of large space, and high cost per single operation. Here, we report a highly sensitive and selective portable diagnostic platform based on photonic polymerase chain reaction. The thermal cycle is operated by the heat energy converted from the photon energy of light emitting diode (LED) through gold layer. We applied the innovative combination between NHCs linker and thin gold film to our newly developed diagnostic device. The interfacial chemistry of a novel structure, N-heterocyclic carbene (NHC), has a special property under thermal stress by forming covalent bonds with the (111) plane of gold. The stable chemical linker to harsh environmental conditions (temperature, ions, and pH) enabled immobilization of bio-probe or anti-quenching interfacial chemistry for further experiment. We developed a new photonic PCR system for RSV diagnosis using a portable scanner-integrated device by overcoming existing few limitations. Effectiveness evaluation of the photonic PCR system was performed via clinical samples from 100 infected patients, and the copy number trend from photonic PCR showed a correlation with the Ct values from RT–PCR. Statistical analysis was carried out to evaluate the discrimination diagnosis ability between samples positive and negative for SARS-CoV-2, showing a sensitivity of 98%, a selectivity of 99%, and an accuracy of 96.4%. In addition, a high-velocity photonic scanner was introduced for on-site application, and fluorescence images from performing PCR were obtained under 7 min. Finally, photonic-based portable systems, e.g., the photonic PCR-integrated high-velocity scanner, can be used to carry out DNA amplification and fluorescence measurements within 15 min. Based on these results, portable scanner-integrated photonic PCR can be utilized on-site in molecular diagnosis of various diagnoses.
References
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| Keywords | N-heterocyclic Carbene, photonic PCR |
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