Speaker
Description
Near-infrared fluorescence-guided surgery using cancer-specific tracers is a promising approach to balance the radicality and quality of life after surgery. Carcinoembryonic antigen (CEA) is a potential target due to its high expression in various digestive cancers, including gastric cancer (GC). This study aims to assess the feasibility of the anti-CEA chimeric monoclonal antibody-dye conjugate emitting at 705 nm, for cancer-specific intraoperative imaging using xenograft mouse models with human cancer cell lines. Four representative human gastric cancer cell lines with CEA expression differences were selected. Selective binding of SGM-101 to CEA on the surface of cancer cells was found using fluorescence-activated cell sorting analysis and immunocytochemistry. in vivo imaging system was used for imaging after intravenous administration of SGM-101 to the subcutaneous implanted models. According to the distribution of this, the highest fluorescence signal was observed 48 hours after injection in a CEA density-dependent manner. Extracted tissue at peak detection time showed a micro-distribution of SGM-101 corresponding to CEA expression evaluated by Immunohistochemistry (IHC). A peritoneal seeding model was created by injecting 85As2mLuc gastric cancer cell line, which expresses both CEA and luciferase activity, into the peritoneal cavity of BALB/c-nu mice. Fluorescence imaging conducted 48 hours after intravenous (IV) administration of SGM-101 could sensitively distinguish nodules suspected of seeding, as confirmed by luminescence imaging and pathologic evaluation. This study confirms the potential of SGM-101 as a patient-tailored surgery tool such as fluorescence-guided navigation, which can identify the metastatic pattern of gastric cancer cells during surgical operation.
References
- Jeong K, Kong SH, Bae SW, Park CR, Berlth F, Shin JH, Lee YS, Youn H, Koo E, Suh YS, Park DJ, Lee HJ, Yang HK. Evaluation of Near-infrared Fluorescence-conjugated Peptides for Visualization of Human Epidermal Receptor 2-overexpressed Gastric Cancer. J Gastric Cancer. 2021;21(2):191-202.
- Gutowski M, Framery B, Boonstra MC, Garambois V, Quenet F, Dumas K, Scherninski F, Cailler F, Vahrmeijer AL, Pèlegrin A. SGM-101: An innovative near-infrared dye-antibody conjugate that targets CEA for fluorescence-guided surgery. Surg Oncol. 2017;26(2):153-62.
- Boogerd LS, Hoogstins CE, Schaap DP, Kusters M, Handgraaf HJ, van der Valk MJ, Hilling DE, Holman FA, Peeters KC, Mieog JSD. Safety and effectiveness of SGM-101, a fluorescent antibody targeting carcinoembryonic antigen, for intraoperative detection of colorectal cancer: a dose-escalation pilot study. The Lancet Gastroenterology & Hepatology. 2018;3(3):181-91.
| Keywords | Fluorescence labeled Chimeric Monoclonal Antibody; Stomach Neoplasm; Carcinoembryonic Antigen; Cancer-specific Imaging; Fluorescence-guided Surgery |
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